Comet parameter: search_enzyme_number

  • The search enzyme is specified by this parameter.
  • The list of search enzymes is specified at the end of the comet.params file beginning with the line [COMET_ENZYME_INFO]. The actual enzyme list and digestion parameters are read here. So one can edit/add/delete enzyme definitions simply be changing the enzyme information.
  • This parameter works in conjection with the num_enzyme_termini parameter to define the cleavage rule for fully-digested vs. semi-digested search options.
  • This parameter works in conjection with the allowed_missed_cleavage parameter to define the miss cleavage rule.

    Example:
    search_enzyme_number = 0     typically no-enzyme
    search_enzyme_number = 1     typically trypsin